Tissue culture protocols from protocols.io:
Primary Human Fibroblast Cell Culture
DNA extraction from whole blood using simple salting out procedure
Protocol for CODEX fixation steps and primary antibody staining for iPSC-derived neurons
Primary Human Fibroblast Cell Culture
DNA extraction from whole blood using simple salting out procedure
Protocol for CODEX fixation steps and primary antibody staining for iPSC-derived neurons
Bio-Protocols:
Automated 384-well SYBR Green Expression Array for Optimization of Human Induced Pluripotent Stem Cell Differentiation
Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
Video protocols (Schuele lab):
Skin Biopsy Processing Video Tutorial
Stem Cell Passaging Video
JOVE Protocols from Schuele lab:
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Links:
Catapult Cell Confluency Tool - for visual estimation of cell confluency
Automated 384-well SYBR Green Expression Array for Optimization of Human Induced Pluripotent Stem Cell Differentiation
Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
Video protocols (Schuele lab):
Skin Biopsy Processing Video Tutorial
Stem Cell Passaging Video
JOVE Protocols from Schuele lab:
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Links:
Catapult Cell Confluency Tool - for visual estimation of cell confluency